UNL is based in the province of Santa Fe, in the central area of Argentina. The university’s main building is located at 2750 Bv. Pellegrini Street, in the city of Santa Fe, but the university also has schools, institutes and faculties in nearby cities, namely Esperanza, Reconquista, Avellaneda, Gálvez, Rafaela and Sunchales.

The city of Santa Fe is the historical site, the “center of gravity” of the university since it is here where most academic units can be found. The city of Esperanza is the link between the university and the rural cluster, whereas the cities of Reconquista, Avellaneda, Gálvez, Rafaela and Sunchales are growing into new areas of social and educational development with a strong regional impact.